polyclonal antibodies against cyclin d 1 Search Results


rabbit polyclonal antibody against cyclin d1 144418  (US Biological Life Sciences)
90
US Biological Life Sciences rabbit polyclonal antibody against cyclin d1 144418
Kaiso depletion attenuates the proliferation of TNBC cells. Growth curve ( a ), MTT assays ( b ) and colony formation assays ( c ) were conducted on Ctrl and Kaiso-depleted (sh-K1 and sh-K2, hereafter referred to as sh-K) cells, and reveal that Kaiso depletion inhibits proliferation of MDA-231 and Hs578T cells. ( d ) Soft agar assays were also conducted on Ctrl, sh-K1 and sh-K2 MDA-231 and Hs578T cells and revealed that Kaiso-depletion diminished anchorage-independent growth of MDA-231 but not Hs578T cells. ( e ) Kaiso depletion resulted in decreased c-Myc and <t>cyclin</t> <t>D1</t> expression in MDA-231 and Hs578T cells as detected by IB analysis and densitometry analysis. The reduction in c-Myc levels in response to Kaiso depletion was more significant in MDA-231 compared to Hs578T cells. GraphPad Prism software was used to generate graphs and for all statistical calculations. Data shown are representative of three independent experiments. * P <0.05, ** P <0.01 and *** P <0.001
Rabbit Polyclonal Antibody Against Cyclin D1 144418, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibodies+against+cyclin+d+1/rabbit+polyclonal+antibody+against+cyclin+d1+144418/pmc05386582-145-32-38
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against cyclin d1 144418 - by Bioz Stars, 2026-10
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cdk4/6 inhibitor  (Mitotix Inc)
90
Mitotix Inc cdk4/6 inhibitor
Kaiso depletion attenuates the proliferation of TNBC cells. Growth curve ( a ), MTT assays ( b ) and colony formation assays ( c ) were conducted on Ctrl and Kaiso-depleted (sh-K1 and sh-K2, hereafter referred to as sh-K) cells, and reveal that Kaiso depletion inhibits proliferation of MDA-231 and Hs578T cells. ( d ) Soft agar assays were also conducted on Ctrl, sh-K1 and sh-K2 MDA-231 and Hs578T cells and revealed that Kaiso-depletion diminished anchorage-independent growth of MDA-231 but not Hs578T cells. ( e ) Kaiso depletion resulted in decreased c-Myc and <t>cyclin</t> <t>D1</t> expression in MDA-231 and Hs578T cells as detected by IB analysis and densitometry analysis. The reduction in c-Myc levels in response to Kaiso depletion was more significant in MDA-231 compared to Hs578T cells. GraphPad Prism software was used to generate graphs and for all statistical calculations. Data shown are representative of three independent experiments. * P <0.05, ** P <0.01 and *** P <0.001
Cdk4/6 Inhibitor, supplied by Mitotix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibodies+against+cyclin+d+1/rabbit+polyclonal+antibodies+directed+against+human+cdk4+and+cyclin+d1/10__1158_slash_2159___8290__cd___15___0894-85-19-47
Average 90 stars, based on 1 article reviews
cdk4/6 inhibitor - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Kaiso depletion attenuates the proliferation of TNBC cells. Growth curve ( a ), MTT assays ( b ) and colony formation assays ( c ) were conducted on Ctrl and Kaiso-depleted (sh-K1 and sh-K2, hereafter referred to as sh-K) cells, and reveal that Kaiso depletion inhibits proliferation of MDA-231 and Hs578T cells. ( d ) Soft agar assays were also conducted on Ctrl, sh-K1 and sh-K2 MDA-231 and Hs578T cells and revealed that Kaiso-depletion diminished anchorage-independent growth of MDA-231 but not Hs578T cells. ( e ) Kaiso depletion resulted in decreased c-Myc and cyclin D1 expression in MDA-231 and Hs578T cells as detected by IB analysis and densitometry analysis. The reduction in c-Myc levels in response to Kaiso depletion was more significant in MDA-231 compared to Hs578T cells. GraphPad Prism software was used to generate graphs and for all statistical calculations. Data shown are representative of three independent experiments. * P <0.05, ** P <0.01 and *** P <0.001

Journal: Cell Death & Disease

Article Title: Kaiso depletion attenuates the growth and survival of triple negative breast cancer cells

doi: 10.1038/cddis.2017.92

Figure Lengend Snippet: Kaiso depletion attenuates the proliferation of TNBC cells. Growth curve ( a ), MTT assays ( b ) and colony formation assays ( c ) were conducted on Ctrl and Kaiso-depleted (sh-K1 and sh-K2, hereafter referred to as sh-K) cells, and reveal that Kaiso depletion inhibits proliferation of MDA-231 and Hs578T cells. ( d ) Soft agar assays were also conducted on Ctrl, sh-K1 and sh-K2 MDA-231 and Hs578T cells and revealed that Kaiso-depletion diminished anchorage-independent growth of MDA-231 but not Hs578T cells. ( e ) Kaiso depletion resulted in decreased c-Myc and cyclin D1 expression in MDA-231 and Hs578T cells as detected by IB analysis and densitometry analysis. The reduction in c-Myc levels in response to Kaiso depletion was more significant in MDA-231 compared to Hs578T cells. GraphPad Prism software was used to generate graphs and for all statistical calculations. Data shown are representative of three independent experiments. * P <0.05, ** P <0.01 and *** P <0.001

Article Snippet: Overnight incubations were performed at 4 °C using the following primary antibodies at their respective dilutions; Kaiso-specific rabbit polyclonal (gift from Dr. Reynolds; 1:5000), mouse monoclonal antibody against c-Myc (SantaCruz (9E10); 1:500), rabbit polyclonal antibody against Cyclin D1 (US Biological (144418); 1:5000), p120 ctn -15D2 specific mouse monoclonal (gift from Dr. Reynolds; 1:1000 ), Bax-specific rabbit monoclonal (1:500; CST-5023), PUMA-specific rabbit monoclonal (1:500; CST-12450), p53-specific rabbit polyclonal (1:2000; Abcam-ab32049), cleaved PARP-specific rabbit monoclonal (1:1000; CST-5625), BRCA1-specific rabbit polyclonal (1:2000; Abcam-ab131360) and mouse anti- β -actin monoclonal (1:50 000; Sigma Aldrich).

Techniques: Expressing, Software

Kaiso-depleted MDA-231 cells exhibit delayed tumor onset in mouse xenografts. ( a ) Kaiso-depleted MDA-231 xenografts (sh-K) are delayed ~3 weeks in tumor onset and development compared to control (Ctrl) MDA-231 xenografted tumors as seen by time-course analysis of the tumor volume of Ctrl and sh-K MDA-231 xenografted cells. ( b ) IHC-stained images of MDA-231 xenograft tissues with Ki-67 and PCNA antibodies show a marked decrease in proliferating cells in MDA-231 Kaiso-depleted tumor tissues as indicated by the reduced expression of the proliferation markers Ki-67 and PCNA. ( c and d ) IHC-stained images of MDA-231 xenograft tissues with c-Myc and Cyclin D1 antibodies show that Kaiso-depletion results in reduced numbers of c-Myc and cyclin-D1 stained cells and reduced staining intensity. Representative images shown from 3 or more independent experiments

Journal: Cell Death & Disease

Article Title: Kaiso depletion attenuates the growth and survival of triple negative breast cancer cells

doi: 10.1038/cddis.2017.92

Figure Lengend Snippet: Kaiso-depleted MDA-231 cells exhibit delayed tumor onset in mouse xenografts. ( a ) Kaiso-depleted MDA-231 xenografts (sh-K) are delayed ~3 weeks in tumor onset and development compared to control (Ctrl) MDA-231 xenografted tumors as seen by time-course analysis of the tumor volume of Ctrl and sh-K MDA-231 xenografted cells. ( b ) IHC-stained images of MDA-231 xenograft tissues with Ki-67 and PCNA antibodies show a marked decrease in proliferating cells in MDA-231 Kaiso-depleted tumor tissues as indicated by the reduced expression of the proliferation markers Ki-67 and PCNA. ( c and d ) IHC-stained images of MDA-231 xenograft tissues with c-Myc and Cyclin D1 antibodies show that Kaiso-depletion results in reduced numbers of c-Myc and cyclin-D1 stained cells and reduced staining intensity. Representative images shown from 3 or more independent experiments

Article Snippet: Overnight incubations were performed at 4 °C using the following primary antibodies at their respective dilutions; Kaiso-specific rabbit polyclonal (gift from Dr. Reynolds; 1:5000), mouse monoclonal antibody against c-Myc (SantaCruz (9E10); 1:500), rabbit polyclonal antibody against Cyclin D1 (US Biological (144418); 1:5000), p120 ctn -15D2 specific mouse monoclonal (gift from Dr. Reynolds; 1:1000 ), Bax-specific rabbit monoclonal (1:500; CST-5023), PUMA-specific rabbit monoclonal (1:500; CST-12450), p53-specific rabbit polyclonal (1:2000; Abcam-ab32049), cleaved PARP-specific rabbit monoclonal (1:1000; CST-5625), BRCA1-specific rabbit polyclonal (1:2000; Abcam-ab131360) and mouse anti- β -actin monoclonal (1:50 000; Sigma Aldrich).

Techniques: Control, Staining, Expressing

Schematic diagram of proposed model for Kaiso's role in TNBC. ( a ) Kaiso interacts with both wt p53 and mutant p53 in BCa cells and this differential interaction may modulate Kaiso's function in apoptosis. ( b ) In TNBC cells lacking wt p53 (but expressing mutant p53), Kaiso might directly or indirectly inhibit the activation of the pro-apoptotic genes Bax and PUMA, which leads to tumor survival. However, Kaiso's inhibitory effect on Bax protein expression may be attenuated by Kaiso interaction with other proteins like p120 ctn . Kaiso may also activate c-Myc, Cyclin D1 and BRCA1 expression independently or in collaboration with other cofactors in TNBC cells, which would also promote TNBC cell growth and survival

Journal: Cell Death & Disease

Article Title: Kaiso depletion attenuates the growth and survival of triple negative breast cancer cells

doi: 10.1038/cddis.2017.92

Figure Lengend Snippet: Schematic diagram of proposed model for Kaiso's role in TNBC. ( a ) Kaiso interacts with both wt p53 and mutant p53 in BCa cells and this differential interaction may modulate Kaiso's function in apoptosis. ( b ) In TNBC cells lacking wt p53 (but expressing mutant p53), Kaiso might directly or indirectly inhibit the activation of the pro-apoptotic genes Bax and PUMA, which leads to tumor survival. However, Kaiso's inhibitory effect on Bax protein expression may be attenuated by Kaiso interaction with other proteins like p120 ctn . Kaiso may also activate c-Myc, Cyclin D1 and BRCA1 expression independently or in collaboration with other cofactors in TNBC cells, which would also promote TNBC cell growth and survival

Article Snippet: Overnight incubations were performed at 4 °C using the following primary antibodies at their respective dilutions; Kaiso-specific rabbit polyclonal (gift from Dr. Reynolds; 1:5000), mouse monoclonal antibody against c-Myc (SantaCruz (9E10); 1:500), rabbit polyclonal antibody against Cyclin D1 (US Biological (144418); 1:5000), p120 ctn -15D2 specific mouse monoclonal (gift from Dr. Reynolds; 1:1000 ), Bax-specific rabbit monoclonal (1:500; CST-5023), PUMA-specific rabbit monoclonal (1:500; CST-12450), p53-specific rabbit polyclonal (1:2000; Abcam-ab32049), cleaved PARP-specific rabbit monoclonal (1:1000; CST-5625), BRCA1-specific rabbit polyclonal (1:2000; Abcam-ab131360) and mouse anti- β -actin monoclonal (1:50 000; Sigma Aldrich).

Techniques: Mutagenesis, Expressing, Activation Assay